Inhibitory postsynaptic currents at Aplysia cholinergic synapses: Effects of permeant anions and depressant drugs
D. J. Adams, P. W. Gage, O. P. Hamill
Inhibitory postsynaptic potentials (i.p.s.ps) and, under voltage-clamp conditions, inhibitory postsynaptic currents (i.p.s.cs) were recorded in neurons in buccal ganglia of Aplysia juliana. the decay of i.p.s.cs was exponential with a single time constant, τ, which decreased with membrane depolarization. In external solutions containing iodide or bromide ions instead of chloride ions, τ varied according to the sequence τ(I)>τ(Br)>τ(Cl), and the voltage sensitivity of τ was altered. In iodide solution, the voltage sensitivity of τ was reversed. Furthermore, the foreign halides depressed the peak current amplitude and shifted the reversal (zero-current) potential to more positive membrane potentials. In low concentrations of sodium pentobarbitone (100-200 μM), the decay of i.p.s.cs became biphasic. Increasing drug concentration and membrane hyperpolarization had differential effects on the rates and relative amplitudes of the two components of i.p.s.c. decay. Octanol (0.5-1 mM) reduced the amplitude of i.p.s.ps and increased the rate of decay of i.p.s.cs without changing the voltage sensitivity of τ. The effect of foreign halides and barbiturates on i.p.s.c. decay were interpreted in terms of a reaction between the anion and an ion-binding site(s) associated with the anion-selective channel, which affects the probability of anions entering the channel and normal channel closure.
Proceedings of the Royal Society of London - Biological Sciences
https://doi.org/10.1098/rspb.1982.0015
Agricultural and Biological Sciences(all)
10.1098/rspb.1982.0015
Dive into the research topics of 'Inhibitory postsynaptic currents at Aplysia cholinergic synapses: Effects of permeant anions and depressant drugs'. Together they form a unique fingerprint.
Aplysia Medicine & Life Sciences 100%
Depressant Chemical Compounds 98%
Inhibitory Postsynaptic Potentials Medicine & Life Sciences 93%
Cholinergic Chemical Compounds 92%
synapse Agriculture & Biology 82%
cholinergic agents Agriculture & Biology 79%
Anions Medicine & Life Sciences 78%
anions Agriculture & Biology 73%
Adams, D. J., Gage, P. W., & Hamill, O. P. (1982). Inhibitory postsynaptic currents at Aplysia cholinergic synapses: Effects of permeant anions and depressant drugs. Proceedings of the Royal Society of London - Biological Sciences, 214(1196), 335-350. https://doi.org/10.1098/rspb.1982.0015
Inhibitory postsynaptic currents at Aplysia cholinergic synapses : Effects of permeant anions and depressant drugs. / Adams, D. J.; Gage, P. W.; Hamill, O. P.
In: Proceedings of the Royal Society of London - Biological Sciences, Vol. 214, No. 1196, 01.01.1982, p. 335-350.
Adams, DJ, Gage, PW & Hamill, OP 1982, 'Inhibitory postsynaptic currents at Aplysia cholinergic synapses: Effects of permeant anions and depressant drugs', Proceedings of the Royal Society of London - Biological Sciences, vol. 214, no. 1196, pp. 335-350. https://doi.org/10.1098/rspb.1982.0015
Adams DJ, Gage PW, Hamill OP. Inhibitory postsynaptic currents at Aplysia cholinergic synapses: Effects of permeant anions and depressant drugs. Proceedings of the Royal Society of London - Biological Sciences. 1982 Jan 1;214(1196):335-350. https://doi.org/10.1098/rspb.1982.0015
Adams, D. J. ; Gage, P. W. ; Hamill, O. P. / Inhibitory postsynaptic currents at Aplysia cholinergic synapses : Effects of permeant anions and depressant drugs. In: Proceedings of the Royal Society of London - Biological Sciences. 1982 ; Vol. 214, No. 1196. pp. 335-350.
@article{7c363d555b884544884d58796a9c1dac,
title = "Inhibitory postsynaptic currents at Aplysia cholinergic synapses: Effects of permeant anions and depressant drugs",
abstract = "Inhibitory postsynaptic potentials (i.p.s.ps) and, under voltage-clamp conditions, inhibitory postsynaptic currents (i.p.s.cs) were recorded in neurons in buccal ganglia of Aplysia juliana. the decay of i.p.s.cs was exponential with a single time constant, τ, which decreased with membrane depolarization. In external solutions containing iodide or bromide ions instead of chloride ions, τ varied according to the sequence τ(I)>τ(Br)>τ(Cl), and the voltage sensitivity of τ was altered. In iodide solution, the voltage sensitivity of τ was reversed. Furthermore, the foreign halides depressed the peak current amplitude and shifted the reversal (zero-current) potential to more positive membrane potentials. In low concentrations of sodium pentobarbitone (100-200 μM), the decay of i.p.s.cs became biphasic. Increasing drug concentration and membrane hyperpolarization had differential effects on the rates and relative amplitudes of the two components of i.p.s.c. decay. Octanol (0.5-1 mM) reduced the amplitude of i.p.s.ps and increased the rate of decay of i.p.s.cs without changing the voltage sensitivity of τ. The effect of foreign halides and barbiturates on i.p.s.c. decay were interpreted in terms of a reaction between the anion and an ion-binding site(s) associated with the anion-selective channel, which affects the probability of anions entering the channel and normal channel closure.",
author = "Adams, {D. J.} and Gage, {P. W.} and Hamill, {O. P.}",
doi = "10.1098/rspb.1982.0015",
journal = "Philosophical Transactions of the Royal Society B: Biological Sciences",
publisher = "Royal Society of London",
T1 - Inhibitory postsynaptic currents at Aplysia cholinergic synapses
T2 - Effects of permeant anions and depressant drugs
AU - Adams, D. J.
AU - Gage, P. W.
AU - Hamill, O. P.
N2 - Inhibitory postsynaptic potentials (i.p.s.ps) and, under voltage-clamp conditions, inhibitory postsynaptic currents (i.p.s.cs) were recorded in neurons in buccal ganglia of Aplysia juliana. the decay of i.p.s.cs was exponential with a single time constant, τ, which decreased with membrane depolarization. In external solutions containing iodide or bromide ions instead of chloride ions, τ varied according to the sequence τ(I)>τ(Br)>τ(Cl), and the voltage sensitivity of τ was altered. In iodide solution, the voltage sensitivity of τ was reversed. Furthermore, the foreign halides depressed the peak current amplitude and shifted the reversal (zero-current) potential to more positive membrane potentials. In low concentrations of sodium pentobarbitone (100-200 μM), the decay of i.p.s.cs became biphasic. Increasing drug concentration and membrane hyperpolarization had differential effects on the rates and relative amplitudes of the two components of i.p.s.c. decay. Octanol (0.5-1 mM) reduced the amplitude of i.p.s.ps and increased the rate of decay of i.p.s.cs without changing the voltage sensitivity of τ. The effect of foreign halides and barbiturates on i.p.s.c. decay were interpreted in terms of a reaction between the anion and an ion-binding site(s) associated with the anion-selective channel, which affects the probability of anions entering the channel and normal channel closure.
AB - Inhibitory postsynaptic potentials (i.p.s.ps) and, under voltage-clamp conditions, inhibitory postsynaptic currents (i.p.s.cs) were recorded in neurons in buccal ganglia of Aplysia juliana. the decay of i.p.s.cs was exponential with a single time constant, τ, which decreased with membrane depolarization. In external solutions containing iodide or bromide ions instead of chloride ions, τ varied according to the sequence τ(I)>τ(Br)>τ(Cl), and the voltage sensitivity of τ was altered. In iodide solution, the voltage sensitivity of τ was reversed. Furthermore, the foreign halides depressed the peak current amplitude and shifted the reversal (zero-current) potential to more positive membrane potentials. In low concentrations of sodium pentobarbitone (100-200 μM), the decay of i.p.s.cs became biphasic. Increasing drug concentration and membrane hyperpolarization had differential effects on the rates and relative amplitudes of the two components of i.p.s.c. decay. Octanol (0.5-1 mM) reduced the amplitude of i.p.s.ps and increased the rate of decay of i.p.s.cs without changing the voltage sensitivity of τ. The effect of foreign halides and barbiturates on i.p.s.c. decay were interpreted in terms of a reaction between the anion and an ion-binding site(s) associated with the anion-selective channel, which affects the probability of anions entering the channel and normal channel closure.
U2 - 10.1098/rspb.1982.0015
DO - 10.1098/rspb.1982.0015
JO - Philosophical Transactions of the Royal Society B: Biological Sciences
JF - Philosophical Transactions of the Royal Society B: Biological Sciences