Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation
A. J. Cheline, A Hari Reddi, R. Bruce Martin
The responses of bone cells to skeletal loading are clearly an important factor in bone biology, but much remains to be learned about the role of these responses in skeletal development, maintenance, and tissue repair. Bone morphogenetic proteins (BMPs) are key regulators of bone formation. We examined the effect of BMP-7 on periosteal and endosteal bone formation in response to increased mechanical loading using the rat tibial bending model. Female Sprague-Dawley rats were divided into four groups of six rats each. Three groups received four point bending loading at 60 N force; the fourth group received sham loading at the same force. The right tibia received 36 cycles of loading on Monday, Wednesday, and Friday for 2 weeks; the left tibia served as a nonloaded control. Just prior to loading, the three loaded groups were injected intraperitoneally with vehicle only or 10 μg/kg or 100 μg/kg of recombinant human BMP-7. Half the sham group received vehicle, and half were given 100 μg/kg of BMP-7. Bone forming surfaces were labeled twice in vivo with calcein, and histomorphometry was performed to quantify periosteal and endosteal bone formation in the loaded and control tibiae. BMP-7 had no effect on periosteal or endosteal bone formation in control or sham-loaded tibiae. Loading produced significantly more woven bone on the periosteal surface than sham loading, but BMP-7 treatment had no effect on this response. Endosteal bone formation was entirely lamellar, and loading (but not sham loading) increased the endosteal mineral apposition and bone formation rates. The higher BMP-7 dose more than doubled the load-induced increase in endosteal lamellar bone formation rate, primarily by increasing the amount of bone forming surface.
Bone morphogenetic protein-7/osteogenic protein-1 (BMP-7/OP-1)
Mechanical adaptation
Dive into the research topics of 'Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation'. Together they form a unique fingerprint.
Bone Morphogenetic Protein 7 Medicine & Life Sciences 100%
Tibia Medicine & Life Sciences 39%
human BMP7 protein Medicine & Life Sciences 17%
fluorexon Medicine & Life Sciences 14%
Bone Morphogenetic Proteins Medicine & Life Sciences 12%
Minerals Medicine & Life Sciences 9%
Cheline, A. J., Reddi, A. H., & Martin, R. B. (2002). Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation. Bone, 31(5), 570-574. https://doi.org/10.1016/S8756-3282(02)00865-7
Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation. / Cheline, A. J.; Reddi, A Hari; Martin, R. Bruce.
In: Bone, Vol. 31, No. 5, 01.11.2002, p. 570-574.
Cheline, AJ, Reddi, AH & Martin, RB 2002, 'Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation', Bone, vol. 31, no. 5, pp. 570-574. https://doi.org/10.1016/S8756-3282(02)00865-7
Cheline AJ, Reddi AH, Martin RB. Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation. Bone. 2002 Nov 1;31(5):570-574. https://doi.org/10.1016/S8756-3282(02)00865-7
Cheline, A. J. ; Reddi, A Hari ; Martin, R. Bruce. / Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation. In: Bone. 2002 ; Vol. 31, No. 5. pp. 570-574.
@article{fca15148229e490ba1b1fe720722354d,
title = "Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation",
abstract = "The responses of bone cells to skeletal loading are clearly an important factor in bone biology, but much remains to be learned about the role of these responses in skeletal development, maintenance, and tissue repair. Bone morphogenetic proteins (BMPs) are key regulators of bone formation. We examined the effect of BMP-7 on periosteal and endosteal bone formation in response to increased mechanical loading using the rat tibial bending model. Female Sprague-Dawley rats were divided into four groups of six rats each. Three groups received four point bending loading at 60 N force; the fourth group received sham loading at the same force. The right tibia received 36 cycles of loading on Monday, Wednesday, and Friday for 2 weeks; the left tibia served as a nonloaded control. Just prior to loading, the three loaded groups were injected intraperitoneally with vehicle only or 10 μg/kg or 100 μg/kg of recombinant human BMP-7. Half the sham group received vehicle, and half were given 100 μg/kg of BMP-7. Bone forming surfaces were labeled twice in vivo with calcein, and histomorphometry was performed to quantify periosteal and endosteal bone formation in the loaded and control tibiae. BMP-7 had no effect on periosteal or endosteal bone formation in control or sham-loaded tibiae. Loading produced significantly more woven bone on the periosteal surface than sham loading, but BMP-7 treatment had no effect on this response. Endosteal bone formation was entirely lamellar, and loading (but not sham loading) increased the endosteal mineral apposition and bone formation rates. The higher BMP-7 dose more than doubled the load-induced increase in endosteal lamellar bone formation rate, primarily by increasing the amount of bone forming surface.",
keywords = "Bending, Bone formation, Bone morphogenetic protein-7/osteogenic protein-1 (BMP-7/OP-1), Mechanical adaptation, Rat",
author = "Cheline, {A. J.} and Reddi, {A Hari} and Martin, {R. Bruce}",
journal = "Bone",
T1 - Bone morphogenetic protein-7 selectively enhances mechanically induced bone formation
AU - Cheline, A. J.
AU - Reddi, A Hari
AU - Martin, R. Bruce
N2 - The responses of bone cells to skeletal loading are clearly an important factor in bone biology, but much remains to be learned about the role of these responses in skeletal development, maintenance, and tissue repair. Bone morphogenetic proteins (BMPs) are key regulators of bone formation. We examined the effect of BMP-7 on periosteal and endosteal bone formation in response to increased mechanical loading using the rat tibial bending model. Female Sprague-Dawley rats were divided into four groups of six rats each. Three groups received four point bending loading at 60 N force; the fourth group received sham loading at the same force. The right tibia received 36 cycles of loading on Monday, Wednesday, and Friday for 2 weeks; the left tibia served as a nonloaded control. Just prior to loading, the three loaded groups were injected intraperitoneally with vehicle only or 10 μg/kg or 100 μg/kg of recombinant human BMP-7. Half the sham group received vehicle, and half were given 100 μg/kg of BMP-7. Bone forming surfaces were labeled twice in vivo with calcein, and histomorphometry was performed to quantify periosteal and endosteal bone formation in the loaded and control tibiae. BMP-7 had no effect on periosteal or endosteal bone formation in control or sham-loaded tibiae. Loading produced significantly more woven bone on the periosteal surface than sham loading, but BMP-7 treatment had no effect on this response. Endosteal bone formation was entirely lamellar, and loading (but not sham loading) increased the endosteal mineral apposition and bone formation rates. The higher BMP-7 dose more than doubled the load-induced increase in endosteal lamellar bone formation rate, primarily by increasing the amount of bone forming surface.
AB - The responses of bone cells to skeletal loading are clearly an important factor in bone biology, but much remains to be learned about the role of these responses in skeletal development, maintenance, and tissue repair. Bone morphogenetic proteins (BMPs) are key regulators of bone formation. We examined the effect of BMP-7 on periosteal and endosteal bone formation in response to increased mechanical loading using the rat tibial bending model. Female Sprague-Dawley rats were divided into four groups of six rats each. Three groups received four point bending loading at 60 N force; the fourth group received sham loading at the same force. The right tibia received 36 cycles of loading on Monday, Wednesday, and Friday for 2 weeks; the left tibia served as a nonloaded control. Just prior to loading, the three loaded groups were injected intraperitoneally with vehicle only or 10 μg/kg or 100 μg/kg of recombinant human BMP-7. Half the sham group received vehicle, and half were given 100 μg/kg of BMP-7. Bone forming surfaces were labeled twice in vivo with calcein, and histomorphometry was performed to quantify periosteal and endosteal bone formation in the loaded and control tibiae. BMP-7 had no effect on periosteal or endosteal bone formation in control or sham-loaded tibiae. Loading produced significantly more woven bone on the periosteal surface than sham loading, but BMP-7 treatment had no effect on this response. Endosteal bone formation was entirely lamellar, and loading (but not sham loading) increased the endosteal mineral apposition and bone formation rates. The higher BMP-7 dose more than doubled the load-induced increase in endosteal lamellar bone formation rate, primarily by increasing the amount of bone forming surface.
KW - Bending
KW - Bone formation
KW - Bone morphogenetic protein-7/osteogenic protein-1 (BMP-7/OP-1)
KW - Mechanical adaptation
KW - Rat
JO - Bone
JF - Bone