Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model
I. J. Liu, A. M. Lee, Martha Kennedy Terris
Purpose: Lack of mucosal regrowth on denuded bowel segments is considered a pre-requisite for successful grafting of cultured urothelial cells for bladder augmentation. This study was designed to establish a technique for clean and complete de-epithelialization of the intestinal mucosa. Materials and Methods: A segment of the small bowel was isolated in six microminipigs. The isolated segment was detubularized and the submucosa injected with HEPES-buffered saline, elevating the mucosa from the underlying tissue and allowing removal with ease. One portion was resected and, along with a portion of the removed mucosa and a sample of the full-thickness bowel, submitted for histologic evaluation. Another portion of the bowel segment remained denuded while the final denuded segment was covered with polyglactin mesh. After 3 days, 1 week, and 3 weeks, specimens from each of the bowel segments were collected for histologic assessment. Immunoperoxidase labeling was performed to confirm the presence or absence of mucosal regrowth. Results: All intestinal segments showed no mucosal regrowth at the 3-day, 1-week, and 3-week incubation periods. Minimal reaction between the denuded bowel segment and the mesh was evident in all of the pigs. Conclusions: In the porcine model, intestinal mucosa can be removed completely using submucosal injection. This technique may be useful in future clinical studies involving bladder augmentation using denuded bowel.
Techniques in Urology
Bladder augmentation
Urinary reservoirs
Fingerprint Dive into the research topics of 'Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model'. Together they form a unique fingerprint.
Intestinal Mucosa Medicine & Life Sciences
Urinary Bladder Medicine & Life Sciences
Mucous Membrane Medicine & Life Sciences
Polyglactin 910 Medicine & Life Sciences
Cultured Cells Medicine & Life Sciences
Clinical Studies Medicine & Life Sciences
Liu, I. J., Lee, A. M., & Terris, M. K. (2001). Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model. Techniques in Urology, 7(1), 70-74.
Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model. / Liu, I. J.; Lee, A. M.; Terris, Martha Kennedy.
In: Techniques in Urology, Vol. 7, No. 1, 28.02.2001, p. 70-74.
Liu, IJ, Lee, AM & Terris, MK 2001, 'Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model', Techniques in Urology, vol. 7, no. 1, pp. 70-74.
Liu IJ, Lee AM, Terris MK. Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model. Techniques in Urology. 2001 Feb 28;7(1):70-74.
Liu, I. J. ; Lee, A. M. ; Terris, Martha Kennedy. / Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model. In: Techniques in Urology. 2001 ; Vol. 7, No. 1. pp. 70-74.
@article{0a5d2ebebf45413aa70ea4964fb7d7a4,
title = "Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model",
abstract = "Purpose: Lack of mucosal regrowth on denuded bowel segments is considered a pre-requisite for successful grafting of cultured urothelial cells for bladder augmentation. This study was designed to establish a technique for clean and complete de-epithelialization of the intestinal mucosa. Materials and Methods: A segment of the small bowel was isolated in six microminipigs. The isolated segment was detubularized and the submucosa injected with HEPES-buffered saline, elevating the mucosa from the underlying tissue and allowing removal with ease. One portion was resected and, along with a portion of the removed mucosa and a sample of the full-thickness bowel, submitted for histologic evaluation. Another portion of the bowel segment remained denuded while the final denuded segment was covered with polyglactin mesh. After 3 days, 1 week, and 3 weeks, specimens from each of the bowel segments were collected for histologic assessment. Immunoperoxidase labeling was performed to confirm the presence or absence of mucosal regrowth. Results: All intestinal segments showed no mucosal regrowth at the 3-day, 1-week, and 3-week incubation periods. Minimal reaction between the denuded bowel segment and the mesh was evident in all of the pigs. Conclusions: In the porcine model, intestinal mucosa can be removed completely using submucosal injection. This technique may be useful in future clinical studies involving bladder augmentation using denuded bowel.",
keywords = "Bladder augmentation, Urinary diversion, Urinary reservoirs",
author = "Liu, {I. J.} and Lee, {A. M.} and Terris, {Martha Kennedy}",
journal = "Techniques in Urology",
publisher = "Raven Press",
T1 - Effectiveness of denuding the intestinal mucosa by submucosal injection in the porcine model
AU - Liu, I. J.
AU - Lee, A. M.
AU - Terris, Martha Kennedy
N2 - Purpose: Lack of mucosal regrowth on denuded bowel segments is considered a pre-requisite for successful grafting of cultured urothelial cells for bladder augmentation. This study was designed to establish a technique for clean and complete de-epithelialization of the intestinal mucosa. Materials and Methods: A segment of the small bowel was isolated in six microminipigs. The isolated segment was detubularized and the submucosa injected with HEPES-buffered saline, elevating the mucosa from the underlying tissue and allowing removal with ease. One portion was resected and, along with a portion of the removed mucosa and a sample of the full-thickness bowel, submitted for histologic evaluation. Another portion of the bowel segment remained denuded while the final denuded segment was covered with polyglactin mesh. After 3 days, 1 week, and 3 weeks, specimens from each of the bowel segments were collected for histologic assessment. Immunoperoxidase labeling was performed to confirm the presence or absence of mucosal regrowth. Results: All intestinal segments showed no mucosal regrowth at the 3-day, 1-week, and 3-week incubation periods. Minimal reaction between the denuded bowel segment and the mesh was evident in all of the pigs. Conclusions: In the porcine model, intestinal mucosa can be removed completely using submucosal injection. This technique may be useful in future clinical studies involving bladder augmentation using denuded bowel.
AB - Purpose: Lack of mucosal regrowth on denuded bowel segments is considered a pre-requisite for successful grafting of cultured urothelial cells for bladder augmentation. This study was designed to establish a technique for clean and complete de-epithelialization of the intestinal mucosa. Materials and Methods: A segment of the small bowel was isolated in six microminipigs. The isolated segment was detubularized and the submucosa injected with HEPES-buffered saline, elevating the mucosa from the underlying tissue and allowing removal with ease. One portion was resected and, along with a portion of the removed mucosa and a sample of the full-thickness bowel, submitted for histologic evaluation. Another portion of the bowel segment remained denuded while the final denuded segment was covered with polyglactin mesh. After 3 days, 1 week, and 3 weeks, specimens from each of the bowel segments were collected for histologic assessment. Immunoperoxidase labeling was performed to confirm the presence or absence of mucosal regrowth. Results: All intestinal segments showed no mucosal regrowth at the 3-day, 1-week, and 3-week incubation periods. Minimal reaction between the denuded bowel segment and the mesh was evident in all of the pigs. Conclusions: In the porcine model, intestinal mucosa can be removed completely using submucosal injection. This technique may be useful in future clinical studies involving bladder augmentation using denuded bowel.
KW - Bladder augmentation
KW - Urinary diversion
KW - Urinary reservoirs
JO - Techniques in Urology
JF - Techniques in Urology