Erasmus Research Institute of Management (ERIM)
Erasmus School of Economics (ESE)
Rotterdam School of Management (RSM)
Erasmus MC: University Medical Center Rotterdam
Erasmus School of Health Policy & Management (ESHPM)
Erasmus School of Law
Public Administration (FSS)
Netherlands Institute for Government (NIG)
Institute for Housing and Urban Development Studies (IHS)
Erasmus School of History, Culture and Communication (ESHCC)
Erasmus School of Social and Behavioural Sciences(ESSB)
Erasmus School of Philosophy (ESPhil)
International Institute of Social Studies (ISS)
Submitting Publications
Department of Pathology /
European Journal of Cancer /
N.P. Malek (N.), J. Casper (J.), L.H.J. Looijenga (Leendert), T. Strohmeyer (T.), H.J. Schmoll, A. Nordheim (A.) and R. Janknecht (R.)
Quantification of additional short arms of chromosome 12 in germ cell turnouts using the polymerase chain reaction
European Journal of Cancer , Volume 33 - Issue 9 p. 1488- 1494
Male germ cell tumours are characterised by the over-representation of 12p sequences, most often in the form of isochromosome i(12p). This study describes the development of a quantitative detection system for additional copies of 12p employing the polymerase chain reaction (PCR). The validity of this method was assessed on two i(12p) containing tumour cell lines in which the number of i(12p) was determined by fluorescence in situ hybridisation. Fourteen primary male germ cell tumours were analysed using the PCR-based method. While 3/8 seminomatous germ cell cancers did not contain any additional 12p, all 6 non-seminomatous tumours did and the severity of the disease correlated with the respective copy number. The ease of the PCR- based method makes it possible for the quantification of additional 12p to become a routine diagnostic and prognostic tool for testicular germ cell tumours, thereby helping to define the role of the i(12p) anomality in larger retrospective studies.
Keywords Carcinoma of uncertain histology, Germ cell tumour, Isochromosome 12p, Molecular diagnosis, Polymerase chain reaction
Persistent URL doi.org/10.1016/S0959-8049(97)00152-4, hdl.handle.net/1765/68913
Journal European Journal of Cancer
Organisation Department of Pathology
Malek, N.P, Casper, J, Looijenga, L.H.J, Strohmeyer, T, Schmoll, H.J, Nordheim, A, & Janknecht, R. (1997). Quantification of additional short arms of chromosome 12 in germ cell turnouts using the polymerase chain reaction. European Journal of Cancer, 33(9), 1488–1494. doi:10.1016/S0959-8049(97)00152-4
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