Simultaneous detection of ferritin and HIV-1 in reactive microglia
M. Yoshioka, P. Shapshak, N. C.J. Sun, S. J. Nelson, A. Svenningsson, L. G. Tate, V. Pardo, L. Resnick
Using ferritin as a marker of reactive microglia, we demonstrated a close association between proliferation of reactive microglia and expression of human immunodeficiency virus type 1 (HIV-1) in brain tissue from autopsied cases of acquired immunodeficiency syndrome (AIDS). An increased number of ferritin-positive reactive microglia was observed in formalin-fixed paraffin-embedded brain sections from all 13 AIDS cases examined. Similar findings were observed in brain tissue from other neurological diseases (subacute sclerosing penencephalitis, herpes simplex encephalitis and multiple sclerosis). Multinucleated giant cells were found in 7 of the AIDS cases which were also intensely labeled for ferritin. Dual-label immunohistochemistry using anti-ferritin and cell-specific markers showed that ferritin-positive cells were distinct from astrocytes, neurons and endothelia using anti-glial fibrillary acidic protein (anti-GFAP), anti-neurofilament protein and Ulex europaeus agglutinin 1, respectively. In 5 AIDS brains, only ferritin-positive cells were shown to contain HIV-1 gp41 antigen using dual-label immunohistochemistry. In addition, HIV-1 RNA was localized in territin-positive reactive microglia but not in GFAP-positive astrocytes using immunohistochemistry combined with in situ hybridization. Ferritin-positive reactive microglia and multinucleated giant cells were colabeled with the microglial marker, Ricinus communis agglutinin 1 (RCA-1). Howerver, RCA-1 also extensively stained resting microglia only a few of which were colabeled for ferritin. The density of ferritin-positive cells was correlated with the presence of HIV-1 RNA-positive cells in AIDS brain. Thus, ferritin immunoreactivity can be used as an activation marker of microglia in archival paraffin sections and reflects the extent of inflammation in HIV-1-infected brain.
Acta neuropathologica
Human immunodeficiency virus type 1
Neuroscience(all)
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Ferritins Medicine & Life Sciences
Microglia Medicine & Life Sciences
Acquired Immunodeficiency Syndrome Medicine & Life Sciences
Ricinus communis agglutinin-1 Medicine & Life Sciences
Immunohistochemistry Medicine & Life Sciences
Yoshioka, M., Shapshak, P., Sun, N. C. J., Nelson, S. J., Svenningsson, A., Tate, L. G., Pardo, V., & Resnick, L. (1992). Simultaneous detection of ferritin and HIV-1 in reactive microglia. Acta neuropathologica, 84(3), 297-306. https://doi.org/10.1007/BF00227823
Simultaneous detection of ferritin and HIV-1 in reactive microglia. / Yoshioka, M.; Shapshak, P.; Sun, N. C.J.; Nelson, S. J.; Svenningsson, A.; Tate, L. G.; Pardo, V.; Resnick, L.
In: Acta neuropathologica, Vol. 84, No. 3, 08.1992, p. 297-306.
Yoshioka, M, Shapshak, P, Sun, NCJ, Nelson, SJ, Svenningsson, A, Tate, LG, Pardo, V & Resnick, L 1992, 'Simultaneous detection of ferritin and HIV-1 in reactive microglia', Acta neuropathologica, vol. 84, no. 3, pp. 297-306. https://doi.org/10.1007/BF00227823
Yoshioka M, Shapshak P, Sun NCJ, Nelson SJ, Svenningsson A, Tate LG et al. Simultaneous detection of ferritin and HIV-1 in reactive microglia. Acta neuropathologica. 1992 Aug;84(3):297-306. https://doi.org/10.1007/BF00227823
Yoshioka, M. ; Shapshak, P. ; Sun, N. C.J. ; Nelson, S. J. ; Svenningsson, A. ; Tate, L. G. ; Pardo, V. ; Resnick, L. / Simultaneous detection of ferritin and HIV-1 in reactive microglia. In: Acta neuropathologica. 1992 ; Vol. 84, No. 3. pp. 297-306.
@article{7f51aa62d71b49b2a9799408947e5190,
title = "Simultaneous detection of ferritin and HIV-1 in reactive microglia",
abstract = "Using ferritin as a marker of reactive microglia, we demonstrated a close association between proliferation of reactive microglia and expression of human immunodeficiency virus type 1 (HIV-1) in brain tissue from autopsied cases of acquired immunodeficiency syndrome (AIDS). An increased number of ferritin-positive reactive microglia was observed in formalin-fixed paraffin-embedded brain sections from all 13 AIDS cases examined. Similar findings were observed in brain tissue from other neurological diseases (subacute sclerosing penencephalitis, herpes simplex encephalitis and multiple sclerosis). Multinucleated giant cells were found in 7 of the AIDS cases which were also intensely labeled for ferritin. Dual-label immunohistochemistry using anti-ferritin and cell-specific markers showed that ferritin-positive cells were distinct from astrocytes, neurons and endothelia using anti-glial fibrillary acidic protein (anti-GFAP), anti-neurofilament protein and Ulex europaeus agglutinin 1, respectively. In 5 AIDS brains, only ferritin-positive cells were shown to contain HIV-1 gp41 antigen using dual-label immunohistochemistry. In addition, HIV-1 RNA was localized in territin-positive reactive microglia but not in GFAP-positive astrocytes using immunohistochemistry combined with in situ hybridization. Ferritin-positive reactive microglia and multinucleated giant cells were colabeled with the microglial marker, Ricinus communis agglutinin 1 (RCA-1). Howerver, RCA-1 also extensively stained resting microglia only a few of which were colabeled for ferritin. The density of ferritin-positive cells was correlated with the presence of HIV-1 RNA-positive cells in AIDS brain. Thus, ferritin immunoreactivity can be used as an activation marker of microglia in archival paraffin sections and reflects the extent of inflammation in HIV-1-infected brain.",
keywords = "Ferritin, Human immunodeficiency virus type 1, Immunohistochemistry, In situ hybridization, Microglia",
author = "M. Yoshioka and P. Shapshak and Sun, {N. C.J.} and Nelson, {S. J.} and A. Svenningsson and Tate, {L. G.} and V. Pardo and L. Resnick",
journal = "Acta Neuropathologica",
T1 - Simultaneous detection of ferritin and HIV-1 in reactive microglia
AU - Yoshioka, M.
AU - Shapshak, P.
AU - Sun, N. C.J.
AU - Nelson, S. J.
AU - Svenningsson, A.
AU - Tate, L. G.
AU - Pardo, V.
AU - Resnick, L.
N2 - Using ferritin as a marker of reactive microglia, we demonstrated a close association between proliferation of reactive microglia and expression of human immunodeficiency virus type 1 (HIV-1) in brain tissue from autopsied cases of acquired immunodeficiency syndrome (AIDS). An increased number of ferritin-positive reactive microglia was observed in formalin-fixed paraffin-embedded brain sections from all 13 AIDS cases examined. Similar findings were observed in brain tissue from other neurological diseases (subacute sclerosing penencephalitis, herpes simplex encephalitis and multiple sclerosis). Multinucleated giant cells were found in 7 of the AIDS cases which were also intensely labeled for ferritin. Dual-label immunohistochemistry using anti-ferritin and cell-specific markers showed that ferritin-positive cells were distinct from astrocytes, neurons and endothelia using anti-glial fibrillary acidic protein (anti-GFAP), anti-neurofilament protein and Ulex europaeus agglutinin 1, respectively. In 5 AIDS brains, only ferritin-positive cells were shown to contain HIV-1 gp41 antigen using dual-label immunohistochemistry. In addition, HIV-1 RNA was localized in territin-positive reactive microglia but not in GFAP-positive astrocytes using immunohistochemistry combined with in situ hybridization. Ferritin-positive reactive microglia and multinucleated giant cells were colabeled with the microglial marker, Ricinus communis agglutinin 1 (RCA-1). Howerver, RCA-1 also extensively stained resting microglia only a few of which were colabeled for ferritin. The density of ferritin-positive cells was correlated with the presence of HIV-1 RNA-positive cells in AIDS brain. Thus, ferritin immunoreactivity can be used as an activation marker of microglia in archival paraffin sections and reflects the extent of inflammation in HIV-1-infected brain.
AB - Using ferritin as a marker of reactive microglia, we demonstrated a close association between proliferation of reactive microglia and expression of human immunodeficiency virus type 1 (HIV-1) in brain tissue from autopsied cases of acquired immunodeficiency syndrome (AIDS). An increased number of ferritin-positive reactive microglia was observed in formalin-fixed paraffin-embedded brain sections from all 13 AIDS cases examined. Similar findings were observed in brain tissue from other neurological diseases (subacute sclerosing penencephalitis, herpes simplex encephalitis and multiple sclerosis). Multinucleated giant cells were found in 7 of the AIDS cases which were also intensely labeled for ferritin. Dual-label immunohistochemistry using anti-ferritin and cell-specific markers showed that ferritin-positive cells were distinct from astrocytes, neurons and endothelia using anti-glial fibrillary acidic protein (anti-GFAP), anti-neurofilament protein and Ulex europaeus agglutinin 1, respectively. In 5 AIDS brains, only ferritin-positive cells were shown to contain HIV-1 gp41 antigen using dual-label immunohistochemistry. In addition, HIV-1 RNA was localized in territin-positive reactive microglia but not in GFAP-positive astrocytes using immunohistochemistry combined with in situ hybridization. Ferritin-positive reactive microglia and multinucleated giant cells were colabeled with the microglial marker, Ricinus communis agglutinin 1 (RCA-1). Howerver, RCA-1 also extensively stained resting microglia only a few of which were colabeled for ferritin. The density of ferritin-positive cells was correlated with the presence of HIV-1 RNA-positive cells in AIDS brain. Thus, ferritin immunoreactivity can be used as an activation marker of microglia in archival paraffin sections and reflects the extent of inflammation in HIV-1-infected brain.
KW - Ferritin
KW - Human immunodeficiency virus type 1
KW - Immunohistochemistry
KW - In situ hybridization
KW - Microglia
JO - Acta Neuropathologica
JF - Acta Neuropathologica