MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells
Kazunori Watanabe, Ryuhei Yamaji, Takashi Ohtsuki
MicroRNAs (miRNAs) belong to a class of small noncoding RNAs that play important roles in the translational regulation of gene expression. A number of miRNAs are known to act as key regulators of diverse processes such as neuronal differentiation. In this study, we have attempted to identify novel miRNAs related to neuronal differentiation via microarray analysis in the human neuronal differentiation model neuroblastoma SH-SY5Y cells. We identified 15 up-regulated and eight down-regulated miRNAs in SH-SY5Y cells treated with all-trans retinoic acid to induce differentiation. We further showed that one of the up-regulated miRNAs, miR-664a-5p, promoted neuronal differentiation of SH-SY5Y cells. These findings enhance our understanding of the miRNAs involved in the process of neurogenesis and, in particular, highlight an important role of miR-664a-5p in SH-SY5Y cell neuronal differentiation. Further studies will be required to confirm the function of miR-664-5p in neuronal development and disease and to identify its relevant target genes.
Genes to Cells
https://doi.org/10.1111/gtc.12559
SH-SY5Y
miR-664a-5p
neuronal differentiation
10.1111/gtc.12559
Dive into the research topics of 'MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells'. Together they form a unique fingerprint.
MicroRNAs Medicine & Life Sciences 100%
Small Untranslated RNA Medicine & Life Sciences 24%
Gene Expression Regulation Medicine & Life Sciences 21%
Neurogenesis Medicine & Life Sciences 20%
Microarray Analysis Medicine & Life Sciences 20%
Tretinoin Medicine & Life Sciences 20%
Neuroblastoma Medicine & Life Sciences 19%
Cell Differentiation Medicine & Life Sciences 16%
Watanabe, K., Yamaji, R., & Ohtsuki, T. (2018). MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. Genes to Cells, 23(3), 225-233. https://doi.org/10.1111/gtc.12559
MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. / Watanabe, Kazunori; Yamaji, Ryuhei; Ohtsuki, Takashi.
In: Genes to Cells, Vol. 23, No. 3, 03.2018, p. 225-233.
Watanabe, K, Yamaji, R & Ohtsuki, T 2018, 'MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells', Genes to Cells, vol. 23, no. 3, pp. 225-233. https://doi.org/10.1111/gtc.12559
Watanabe K, Yamaji R, Ohtsuki T. MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. Genes to Cells. 2018 Mar;23(3):225-233. doi: 10.1111/gtc.12559
Watanabe, Kazunori ; Yamaji, Ryuhei ; Ohtsuki, Takashi. / MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. In: Genes to Cells. 2018 ; Vol. 23, No. 3. pp. 225-233.
@article{815a231e66d14116bc64fb2d9947c42d,
title = "MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells",
abstract = "MicroRNAs (miRNAs) belong to a class of small noncoding RNAs that play important roles in the translational regulation of gene expression. A number of miRNAs are known to act as key regulators of diverse processes such as neuronal differentiation. In this study, we have attempted to identify novel miRNAs related to neuronal differentiation via microarray analysis in the human neuronal differentiation model neuroblastoma SH-SY5Y cells. We identified 15 up-regulated and eight down-regulated miRNAs in SH-SY5Y cells treated with all-trans retinoic acid to induce differentiation. We further showed that one of the up-regulated miRNAs, miR-664a-5p, promoted neuronal differentiation of SH-SY5Y cells. These findings enhance our understanding of the miRNAs involved in the process of neurogenesis and, in particular, highlight an important role of miR-664a-5p in SH-SY5Y cell neuronal differentiation. Further studies will be required to confirm the function of miR-664-5p in neuronal development and disease and to identify its relevant target genes.",
keywords = "SH-SY5Y, miR-664a-5p, microRNA, neuronal differentiation",
author = "Kazunori Watanabe and Ryuhei Yamaji and Takashi Ohtsuki",
note = "Funding Information: This study was supported in part by The Hattori Hokokai Foundation, The Ryobi Teien Foundation to KW and by JSPS KAKENHI (25282232) to TO. We thank Filgen, Inc. for their analysis of miRNA microarrays. We thank Dr D.O. Wang and Wang{\textquoteright}s group (Kyoto University) for prediction of miRNA-664a-5p direct target.",
doi = "10.1111/gtc.12559",
journal = "Genes to Cells",
T1 - MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells
AU - Watanabe, Kazunori
AU - Yamaji, Ryuhei
AU - Ohtsuki, Takashi
N1 - Funding Information: This study was supported in part by The Hattori Hokokai Foundation, The Ryobi Teien Foundation to KW and by JSPS KAKENHI (25282232) to TO. We thank Filgen, Inc. for their analysis of miRNA microarrays. We thank Dr D.O. Wang and Wang's group (Kyoto University) for prediction of miRNA-664a-5p direct target.
N2 - MicroRNAs (miRNAs) belong to a class of small noncoding RNAs that play important roles in the translational regulation of gene expression. A number of miRNAs are known to act as key regulators of diverse processes such as neuronal differentiation. In this study, we have attempted to identify novel miRNAs related to neuronal differentiation via microarray analysis in the human neuronal differentiation model neuroblastoma SH-SY5Y cells. We identified 15 up-regulated and eight down-regulated miRNAs in SH-SY5Y cells treated with all-trans retinoic acid to induce differentiation. We further showed that one of the up-regulated miRNAs, miR-664a-5p, promoted neuronal differentiation of SH-SY5Y cells. These findings enhance our understanding of the miRNAs involved in the process of neurogenesis and, in particular, highlight an important role of miR-664a-5p in SH-SY5Y cell neuronal differentiation. Further studies will be required to confirm the function of miR-664-5p in neuronal development and disease and to identify its relevant target genes.
AB - MicroRNAs (miRNAs) belong to a class of small noncoding RNAs that play important roles in the translational regulation of gene expression. A number of miRNAs are known to act as key regulators of diverse processes such as neuronal differentiation. In this study, we have attempted to identify novel miRNAs related to neuronal differentiation via microarray analysis in the human neuronal differentiation model neuroblastoma SH-SY5Y cells. We identified 15 up-regulated and eight down-regulated miRNAs in SH-SY5Y cells treated with all-trans retinoic acid to induce differentiation. We further showed that one of the up-regulated miRNAs, miR-664a-5p, promoted neuronal differentiation of SH-SY5Y cells. These findings enhance our understanding of the miRNAs involved in the process of neurogenesis and, in particular, highlight an important role of miR-664a-5p in SH-SY5Y cell neuronal differentiation. Further studies will be required to confirm the function of miR-664-5p in neuronal development and disease and to identify its relevant target genes.
KW - SH-SY5Y
KW - miR-664a-5p
KW - microRNA
KW - neuronal differentiation
U2 - 10.1111/gtc.12559
DO - 10.1111/gtc.12559
JO - Genes to Cells
JF - Genes to Cells