Halomonas daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield soil
Gang Wu1, Xiao-Qing Wu1, Ya-Nan Wang2, Chang-Qiao Chi2, Yue-Qin Tang2, Kenji Kida3, Xiao-Lei Wu2, Zhao-Kun Luan1
Affiliations: 1 1State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China 2 2Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, PR China 3 3Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto City, Kumamoto 860-8555, Japan
CorrespondenceXiao-Lei Wu
[email protected]
Ya-Nang Wang
[email protected]
Published: 01 December 2008 https://doi.org/10.1099/ijs.0.65746-0
A Gram-negative, moderately halophilic, short rod-shaped, aerobic bacterium with peritrichous flagellae, strain DQD2-30T, was isolated from a soil sample contaminated with crude oil from the Daqing oilfield in Heilongjiang Province, north-eastern China. The novel strain was capable of growth at NaCl concentrations of 1–15 % (w/v) [optimum at 5–10 % (w/v)]. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Halomonas in the class Gammaproteobacteria; the highest 16S rRNA gene sequence similarities were with Halomonas desiderata DSM 9502T (98.8 %), Halomonas campisalis A4T (96.6 %) and Halomonas gudaonensis CGMCC 1.6133T (95.1 %). The major cellular fatty acids of strain DQD2-30T were C18 : 1 ω7c (43.97 %), C19 : 0 cyclo ω8c (23.37 %) and C16 : 0 (14.83 %). The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q9). The DNA G+C content was 67.0 mol%. The DNA–DNA hybridization values of strain DQD2-30T with the most closely related species of the genus Halomonas were 51.8 %, 28.4 % and 23.5 % for H. desiderata, H. campisalis and H. gudaonensis, respectively. Based on these analyses, strain DQD2-30T (=CGMCC 1.6443T=LMG 23896T) is proposed to represent the type strain of a novel species, Halomonas daqingensis sp. nov.
Keyword(s): DPG, diphosphatidylglycerol , EPS, exopolysaccharides , PE, phosphatidylethanolamine , PG, phosphatidylglycerol and PHB, poly-β-hydroxybutyrate
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