Lung cancer continues to be the leading cause of cancer-related deaths worldwide. Early detection and accurate diagnosis of lung lesions are critical to reduce mortality and there remains a need for a collaborative effort in the discovery of advanced therapeutic approaches for lung cancer. SCIREQ is proud to be a part of the efforts by offering state-of-the art equipment for preclinical studies.
Cancer model generation and advancing therapeutic approaches are important components of preclinical cancer studies. SCIREQ offers both the tools for cancer model generation and to allow the investigation of advancements in surgery as a therapeutic approach in small subjects.
Additionally, localized inhalation drug delivery has generated interest among scientists as a strategy to achieve better activity of therapeutic drugs for the treatment of lung cancer because of site-specificity, low doses, and recipients' compliance.
Advanced lung function measurements
At the preclinical level, when studying tumour progression and phenotypical changes in small subjects, it is important to perform non-terminal surgeries for continued research. The flexiVent is a programmable mechanical ventilator that has become invaluable in many cancer studies involving small subjects. The flexiVent uses the forced oscillation technique (FOT) to probe the mechanical properties of the lungs in anesthetized subjects in detail and in a reproducible manner. The flexiVent has proven to be a gold standard for mechanical ventilation for tracking cancer progression using micro-CT imaging. The programmable aspect of the flexiVent allows advanced gating techniques by enabling image capture during breath-hold, increasing image contrast and resolution.
References & Publications
Longitudinal assessment of lung cancer progression in the mouse using in vivo micro-CT imaging. Namati, Eman, et al. (2010). Medical physics, 37(9), 4793-4805
Individual nodule tracking in micro-CT images of a longitudinal lung cancer mouse model. Rudyanto, Rina D., et al. (2013). Medical image analysis, 17(8),1095-1105.
The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Cronin, S.J.F., et al. (2018). Nature, 563: 564-568
Celecoxib potentially inhibits metastasis of lung cancer promoted by surgery in mice, via suppression of the PGE2-modulated β-catenin pathway. Zhang, S., et al. (2014). Toxicology Letters, 225(2): 201-207
Characterization of Lung Cancer by Amide Proton Transfer (APT) Imaging: An In-Vivo Study in an Orthotopic Mouse Model. Togao, O., et al (2013). PLOS ONE, https://doi.org/10.1371/journal.pone.0077019
Reproducible exposure, precise delivery
The inExpose is a compact and customizable inhalation exposure system. The inExpose has been specifically designed for consistent and precise targeted aerosol delivery by controlling air flow rates and aerosol generation devices. Automated exposure profiles reduce user error and minimize outcome variability. The inExpose ensures that subjects receive repeatable yet consistent exposures throughout experimentation which is useful in cancer model generation and studying anticancer activities of aerosolized therapeutics. When considering inhalation as the route for drug delivery, the inExpose standardizes experimental conditions providing reproducible and relevant animal models. Additionally, the inExpose provides control over experimental conditions beyond typical inhalant parameters, offering a unique testing environment for therapeutics.
Efficacy of Aerosolized Celecoxib Encapsulated Nanostructured Lipid Carrier in Non-small Cell Lung Cancer in Combination with Docetaxel. Patel et al. (2013). Pharm Res. May; 30(5):1435-46.
Efficacy of aerosol therapy of lung cancer correlates with EGFR paralysis induced by AvidinOX-anchored biotinylated Cetuximab. De Santis et al. (2014). Oncotarget. Aug;5(19):9239-55.
In Vitro and In Vivo Assessment of Pulmonary Risk Associated with Exposure to Combustion Generated Fine Particles. Fahmy B et al. (2010). Environ Toxicol Pharmacol. 29:173.
Inhalation delivery of Telmisartan enhances intratumoral distribution of nanoparticles in lung cancer models. Godugu, C., et al. (2013). Journal of Controlled Release, 172(1): 86-95
1,1-Bis (30 -indolyl)-1-(p-substitutedphenyl)methane compounds inhibit lung cancer cell and tumor growth in a metastasis model. Andey, T., et al. (2013). European Journal Of Pharmaceutical Sciences, 50: 227-241
Conscious ventillatory measurements
Respiratory function can be assessed in conscious subjects using various plethysmography techniques. Whole body plethysmography permits a continuous and non-invasive assessment of breathing patterns in conscious subjects. Measurements of respiratory rate, estimated tidal volume, and minute ventilation provide valuable insights into the subject breathing drive and behaviour. Cancer studies using plethysmography techniques evaluate the effects of various gene deletions on respiratory function in knockout vs control populations. Whole body plethysmography is also used to assess animal responsiveness to challenge.
Deletion of the von Hippel–Lindau Gene Causes Sympathoadrenal Cell Death and Impairs Chemoreceptor-Mediated Adaptation to Hypoxia. Macias, D., et al. (2014). EMBO Molecular Medicine 6, no. 12: 1577–92. https://doi.org/10.15252/emmm.201404153.
Ribitol Restores Functionally Glycosylated α-Dystroglycan and Improves Muscle Function in Dystrophic FKRP-Mutant Mice. Cataldi, Marcela P., Peijuan Lu, Anthony Blaeser, and Qi Long Lu. (2018). Nature Communications 9, no. 1: 3448. https://doi.org/10.1038/s41467-018-05990-z.
Involvement of ER Stress, PI3K/AKT Activation, and Lung Fibroblast Proliferation in Bleomycin-Induced Pulmonary Fibrosis. Hsu, Han-Shui, Chen-Chi Liu, Jiun-Han Lin, Tien-Wei Hsu, Jyuan-Wei Hsu, Kelly Su, and Shih-Chieh Hung. (2017). Scientific Reports 7, no. 1: 14272. https://doi.org/10.1038/s41598-017-14612-5.
Request a Free Custom Literature Search
Save time evaluating SCIREQ equipment by having a SCIREQ Application Specialist conduct a custom
literature research. Researchers will receive an email report showing SCIREQ publications relevant to their specific application research area, along with custom equipment recommendations and commentary.
Request Literature Search