Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila
Eugenia C. Olesnicky, Balpreet Bhogal, Elizabeth R. Gavis
The translational regulators Nanos (Nos) and Pumilio (Pum) work together to regulate the morphogenesis of dendritic arborization (da) neurons of the Drosophila larval peripheral nervous system. In contrast, Nos and Pum function in opposition to one another in the neuromuscular junction to regulate the morphogenesis and the electrophysiological properties of synaptic boutons. Neither the cellular functions of Nos and Pum nor their regulatory targets in neuronal morphogenesis are known. Here we show that Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches, in part by regulating the expression of cut and head involution defective. Through an RNA interference screen we uncover a role for the translational co-factor Brain Tumor (Brat) in dendrite morphogenesis of da neurons and demonstrate that Nos, Pum, and Brat interact genetically to regulate dendrite morphogenesis. In the neuromuscular junction, Brat function is most likely specific for Pum in the presynaptic regulation of bouton morphogenesis. Our results reveal how the combinatorial use of co-regulators like Nos, Pum and Brat can diversify their roles in post-transcriptional regulation of gene expression for neuronal morphogenesis.
Dendrite morphogenesis
Neuromuscular junction
Pumilio
Dive into the research topics of 'Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila'. Together they form a unique fingerprint.
Morphogenesis Medicine & Life Sciences 100%
Drosophila Medicine & Life Sciences 92%
Brain Neoplasms Medicine & Life Sciences 52%
Neuronal Plasticity Medicine & Life Sciences 40%
Neuromuscular Junction Medicine & Life Sciences 33%
Dendrites Medicine & Life Sciences 29%
Presynaptic Terminals Medicine & Life Sciences 15%
Olesnicky, E. C., Bhogal, B., & Gavis, E. R. (2012). Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila. Developmental biology, 365(1), 208-218. https://doi.org/10.1016/j.ydbio.2012.02.028
Olesnicky, Eugenia C. ; Bhogal, Balpreet ; Gavis, Elizabeth R. / Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila. In: Developmental biology. 2012 ; Vol. 365, No. 1. pp. 208-218.
@article{63495d65944f49fd99e5502785086486,
title = "Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila",
abstract = "The translational regulators Nanos (Nos) and Pumilio (Pum) work together to regulate the morphogenesis of dendritic arborization (da) neurons of the Drosophila larval peripheral nervous system. In contrast, Nos and Pum function in opposition to one another in the neuromuscular junction to regulate the morphogenesis and the electrophysiological properties of synaptic boutons. Neither the cellular functions of Nos and Pum nor their regulatory targets in neuronal morphogenesis are known. Here we show that Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches, in part by regulating the expression of cut and head involution defective. Through an RNA interference screen we uncover a role for the translational co-factor Brain Tumor (Brat) in dendrite morphogenesis of da neurons and demonstrate that Nos, Pum, and Brat interact genetically to regulate dendrite morphogenesis. In the neuromuscular junction, Brat function is most likely specific for Pum in the presynaptic regulation of bouton morphogenesis. Our results reveal how the combinatorial use of co-regulators like Nos, Pum and Brat can diversify their roles in post-transcriptional regulation of gene expression for neuronal morphogenesis.",
keywords = "Brain tumor, Dendrite morphogenesis, Nanos, Neuromuscular junction, Pumilio",
author = "Olesnicky, {Eugenia C.} and Balpreet Bhogal and Gavis, {Elizabeth R.}",
note = "Funding Information: We thank H. Ashe, A. Brand, N. Brown, I. Davis, W. Grueber, Y.N. Jan, K. Menon, D. St Johnston, R. Wharton, K. Zinn, the Bloomington Drosophila stock center, the Vienna Drosophila RNAi Center, and TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for fly stocks and P. Lasko, H. Steller, and R. Wharton for antibodies. This work was supported by the National Institutes of Health ( F32 HD056779 to E.C.O and R01 GM061107 to E.R.G).",
Olesnicky, EC, Bhogal, B & Gavis, ER 2012, 'Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila', Developmental biology, vol. 365, no. 1, pp. 208-218. https://doi.org/10.1016/j.ydbio.2012.02.028
Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila. / Olesnicky, Eugenia C.; Bhogal, Balpreet; Gavis, Elizabeth R.
T1 - Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila
AU - Olesnicky, Eugenia C.
AU - Bhogal, Balpreet
AU - Gavis, Elizabeth R.
N1 - Funding Information: We thank H. Ashe, A. Brand, N. Brown, I. Davis, W. Grueber, Y.N. Jan, K. Menon, D. St Johnston, R. Wharton, K. Zinn, the Bloomington Drosophila stock center, the Vienna Drosophila RNAi Center, and TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for fly stocks and P. Lasko, H. Steller, and R. Wharton for antibodies. This work was supported by the National Institutes of Health ( F32 HD056779 to E.C.O and R01 GM061107 to E.R.G).
N2 - The translational regulators Nanos (Nos) and Pumilio (Pum) work together to regulate the morphogenesis of dendritic arborization (da) neurons of the Drosophila larval peripheral nervous system. In contrast, Nos and Pum function in opposition to one another in the neuromuscular junction to regulate the morphogenesis and the electrophysiological properties of synaptic boutons. Neither the cellular functions of Nos and Pum nor their regulatory targets in neuronal morphogenesis are known. Here we show that Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches, in part by regulating the expression of cut and head involution defective. Through an RNA interference screen we uncover a role for the translational co-factor Brain Tumor (Brat) in dendrite morphogenesis of da neurons and demonstrate that Nos, Pum, and Brat interact genetically to regulate dendrite morphogenesis. In the neuromuscular junction, Brat function is most likely specific for Pum in the presynaptic regulation of bouton morphogenesis. Our results reveal how the combinatorial use of co-regulators like Nos, Pum and Brat can diversify their roles in post-transcriptional regulation of gene expression for neuronal morphogenesis.
AB - The translational regulators Nanos (Nos) and Pumilio (Pum) work together to regulate the morphogenesis of dendritic arborization (da) neurons of the Drosophila larval peripheral nervous system. In contrast, Nos and Pum function in opposition to one another in the neuromuscular junction to regulate the morphogenesis and the electrophysiological properties of synaptic boutons. Neither the cellular functions of Nos and Pum nor their regulatory targets in neuronal morphogenesis are known. Here we show that Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches, in part by regulating the expression of cut and head involution defective. Through an RNA interference screen we uncover a role for the translational co-factor Brain Tumor (Brat) in dendrite morphogenesis of da neurons and demonstrate that Nos, Pum, and Brat interact genetically to regulate dendrite morphogenesis. In the neuromuscular junction, Brat function is most likely specific for Pum in the presynaptic regulation of bouton morphogenesis. Our results reveal how the combinatorial use of co-regulators like Nos, Pum and Brat can diversify their roles in post-transcriptional regulation of gene expression for neuronal morphogenesis.
KW - Brain tumor
KW - Dendrite morphogenesis
KW - Nanos
KW - Neuromuscular junction
KW - Pumilio
Olesnicky EC, Bhogal B, Gavis ER. Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila. Developmental biology. 2012 May 1;365(1):208-218. doi: 10.1016/j.ydbio.2012.02.028